Hi everyone,
I am new to cell culturing. My supervisor has asked me to find out the medium required to culture squamous cells. I am doing literature search. In the meanwhile I was hoping if I could get help from you guys. Any help will be appreciated.
Thanks.
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3 replies to this topic
#2
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Hmmm, I think it's working
Posted 12 August 2009 - 04:13 PM
Culture from tissue (primary culture) or as a cell line bought/gifted?
If as a cell line, use the medium it is supplied in. As a primary, you may have to try several media and different conditions, and maybe have to get specialist medium in.
If as a cell line, use the medium it is supplied in. As a primary, you may have to try several media and different conditions, and maybe have to get specialist medium in.
#3
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Posted 13 August 2009 - 12:49 AM
More details on the cell line/origin would help.
antibiotic/antimycotic concentrations will have to be high if it is a primary culture.
If you have plenty of sample I would suggest trying a basic DMEM but this is purely bias on my own usages/experience.
antibiotic/antimycotic concentrations will have to be high if it is a primary culture.
If you have plenty of sample I would suggest trying a basic DMEM but this is purely bias on my own usages/experience.
#4
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Posted 16 August 2009 - 08:34 PM
I meant to ask for primary cultures...
