Media for squamous cells
Posted 11 August 2009 - 07:41 PM
I am new to cell culturing. My supervisor has asked me to find out the medium required to culture squamous cells. I am doing literature search. In the meanwhile I was hoping if I could get help from you guys. Any help will be appreciated.
Posted 12 August 2009 - 04:13 PM
If as a cell line, use the medium it is supplied in. As a primary, you may have to try several media and different conditions, and maybe have to get specialist medium in.
Posted 13 August 2009 - 12:49 AM
antibiotic/antimycotic concentrations will have to be high if it is a primary culture.
If you have plenty of sample I would suggest trying a basic DMEM but this is purely bias on my own usages/experience.