Suitable bacterial reporter vectors
Posted 11 August 2009 - 07:45 AM
This may be more of a molecular biology question...
I want to use a reporter plasmid to look for activation of a particular gene promoter in E. coli. I plan to clone the promoter sequence for the GOI into a plasmid upstream of a reporter gene (either GFP or beta-gal i havent decided yet).
Please could anyone recommend suitable plasmids for doing this. pUC18 for example could be suitable for a beta-gal reporter but Im not sure of a suitable GFP containing vector that i could use.
What will be the impact of plasmid copy number? - i assume that high copy number would give me a stronger signal upon activation of the promoter?
All the best,
Posted 11 August 2009 - 09:20 AM
Hope this helps
Posted 25 November 2009 - 09:06 AM
If you are going to screen for promoter-activity on agar-plates the higher the copy number the better because you will be able to find high-level expression clones by eye (even without UVlight).