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Human collagen type I molecular weight


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#1 abohollolo

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Posted 10 August 2009 - 07:45 PM

Hello

I am trying to look for molecular weight for human collagen type I ...but i can`t find

please any help

(i want to do western blotting for collagen type I )

but i dont know where collagen bands should appear

#2 Chakchel

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Posted 10 August 2009 - 10:35 PM

Hi Abohollolo,

monomeric collagen type I consists of alpha-1 and alpha-2 chains.
Alpha-1 is 139 kDa and alpha-2 is 129kDa.
As collagen type I is a triple helix consisting of one alpha-2 chain and two alpha-1 chains, you may also get bands of the dimeric (~270 kDa) and the trimeric form (~400 kDa).
Remember that those chains are cross-linked and can't be broken by typical sample denaturation for SDS-PAGE.

Greetings, Chakchel

#3 abohollolo

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Posted 10 August 2009 - 11:08 PM

Hi Abohollolo,

monomeric collagen type I consists of alpha-1 and alpha-2 chains.
Alpha-1 is 139 kDa and alpha-2 is 129kDa.
As collagen type I is a triple helix consisting of one alpha-2 chain and two alpha-1 chains, you may also get bands of the dimeric (~270 kDa) and the trimeric form (~400 kDa).
Remember that those chains are cross-linked and can't be broken by typical sample denaturation for SDS-PAGE.

Greetings, Chakchel


great thanks ..
so what is the preferable conditions to run sds-page and WB in your experience ?

#4 Chakchel

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Posted 11 August 2009 - 12:40 AM

Hi,
sorry, I don't work on this - the only experience I have is from a lab course a couple of years ago...
I guess we denatured the samples with guanidiniumchloride, which breaks the hydrogen bonds, but I don't have the exact protocol here... :)

Maybe the others know?

Good luck!




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