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extracting protein from trizol


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#1 danjordan

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Posted 10 August 2009 - 12:38 PM

Hello everyone!

I have a doubt concerning protein extraction using trizol. I dont know if anyone had ever used this protocol, but during the procedure, when you have to dissolve the protein pellet in SDS, a huge white pellet is still present at the eppendorf and it seems to be unsoluble. Do anyone have any idea of whats happening?

Thanks!

#2 lab rat

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Posted 12 August 2009 - 11:39 AM

Hi Dan,

If the sample has been washed sufficiently and heating the pellet to 50C during dissolution doesn't work, then the white pellet is probably insoluble material. Just discard it.
42..."An immutable fixed-precision number of unlimited magnitude." <a href="http://en.wikipedia....amming_language)" target="_blank">http://en.wikipedia....amming_language)</a>, accessed 25June2009.

#3 Xanthier

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Posted 22 September 2009 - 08:48 PM

I've been trying to do protein extraction using Trizol as well. I think the huge white pellet is still protein. Has anyone else had experience on dissolving the protein pellet after isolation using Trizol?




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