extracting protein from trizol
Posted 10 August 2009 - 12:38 PM
I have a doubt concerning protein extraction using trizol. I dont know if anyone had ever used this protocol, but during the procedure, when you have to dissolve the protein pellet in SDS, a huge white pellet is still present at the eppendorf and it seems to be unsoluble. Do anyone have any idea of whats happening?
Posted 12 August 2009 - 11:39 AM
If the sample has been washed sufficiently and heating the pellet to 50C during dissolution doesn't work, then the white pellet is probably insoluble material. Just discard it.
Posted 22 September 2009 - 08:48 PM