how to trace single cell movement throughout gastrulation of zebrafish
Posted 10 August 2009 - 08:07 AM
could anyone give me good idea how can I achieve this purpose?
should I use specific fluorescent dye? won't that be diffusing if I inject into fish embryo?
i know somebody used the DMNB-caged fluorescence dextran before but Invitrogen is no longer providing it....
thanks for all of you who's giving me any kind of suggestion!!
Posted 10 August 2009 - 05:27 PM
Posted 11 August 2009 - 02:52 AM
Posted 08 December 2009 - 08:05 PM
1. inject at late cleavage stage on random side of the embryos (with gfp, rfp, rldx, fldx,or whatever with fluorescence), and pick up the embryos at later developmental stages with fluorescence at the desired region.
2. using photoconversion strategy, such as caged dyes or Kaede-GFP, and label by UV irridation at the desired region.
3. transplant the labeled cells to the corresponding region of a recipient.