storing large amounts of PCR mastermix beforehand
Posted 10 August 2009 - 07:02 AM
Posted 10 August 2009 - 11:17 AM
I have stored such mastermixes (even including Taq) overnight at 4'C, without any appreciable reduction in PCR products. I have not been so daring as to store it in -20 or for prolonged periods of time.
Would it be fine to make up large amounts of PCR mastermix, but exclude Taq polymerase, DNA template, and primers? I want to store overnight in the freezer so I can just come in and start the next day or perhaps within that week. Just wondering the length of stability of the mastermix would be over time too?
I guess it also depends upon the robustness of your product specific PCR. But let us know your insights if you try this out at your expense!
Posted 10 August 2009 - 06:03 PM
Do let us know how things go for you. =)
Posted 11 August 2009 - 07:54 AM