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Oxygen diffusion through cell culture medium


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#1 victor.m

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Posted 10 August 2009 - 06:51 AM

Hi all,
I would have a question regarding oxygen diffusion through/in cell culture medium. If I have cells on plate with 2 ml of cell culture medium (surface of the well is 9.5 cm2) and then I put the plate from normoxia to hypoxia (1% of oxygen, 37C is in the hypoxia incubator), how long approximately does it take to reach 1% of oxygen in the cell culture medium (and in cells)?

Thanks.
With regards,
vic

#2 genehunter

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Posted 10 August 2009 - 06:56 AM

Its best to measure it experimentally because there are so many variables involved.

#3 bob1

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Posted 10 August 2009 - 05:18 PM

there are equations that describe the diffusion rate in an ideal system, but as genehunter said, there are lots of variables involved.

http://en.wikipedia....fusion_equation




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