help: bovine Intestinal Epithelial Cell Culture protocol
Posted 07 August 2009 - 07:37 PM
er, in fact, i am a fresh learen in cell culturing, i have to islate and culture bovine Intestinal Epithelial Cell. i know firstly i have to search for some former documents and some protocols, but i found nothing useful. i have to start very soon, so if anyone of you can supply me with some information, i will be very appreciate!
P.S.if it is convenint for you, you can send to my email.,THANKS!
Posted 10 August 2009 - 09:52 PM
Posted 12 August 2009 - 05:36 AM
Thanks for your care. You are quite right, i do want to do some primary cultures.I need the cell to do gene targetting, there is a flow cytometer in my lab.Then do you have some good advices?
Could you provide further details about intentions of the project? I assume these are going to be primary cultures? how specific do you need the cell population? do you have access to a flow cytometer?
p.s. I am sorry to reply so late!
Posted 13 August 2009 - 12:57 AM
Posted 13 August 2009 - 08:47 AM