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How to do viral titre counts?


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#1 cppoo1

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Posted 07 August 2009 - 10:28 AM

Hi all,

Just have a quick question: How does one do a physical viral titer count (particles/mL)? Is it by OD280? Anyone have a protocol?

Thanks very much in advance.

#2 cellcounter

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Posted 10 August 2009 - 11:44 AM

Hi all,

Just have a quick question: How does one do a physical viral titer count (particles/mL)? Is it by OD280? Anyone have a protocol?

Thanks very much in advance.

The second result here has many references for performing actual viral particle counts.

#3 mem

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Posted 18 August 2009 - 02:03 PM

Question: Are you trying to titer a virus that has a known host in culture. If so you can use the plaque assay to determine your titer (here is a method for the plaque assay : http://www.virusecol.../Method 8.html). If your virus does not have a host or if you are just doing total virus counts you can use microscopy (Noble, R.T. and Fuhrman, J.A. 1998. Use of SYBR Green I for rapid epifluoresence counts of marine viruses and bacteria).




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