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should i put reference wavelength?

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#1 wntiong



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Posted 07 August 2009 - 01:06 AM

Hi i just found out that some kit manuals put measurement wavelength (450nm) and reference wavelength (620nm) as well? But some kit they dont mention any reference wavelength. Should i put reference wavelength so to have reference data as well (instead of raw data measured by instrument)? Thanks.

#2 Gerard



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Posted 09 August 2009 - 05:01 AM

The reference wavelenght kan be usefull to detect scratches or airbubbles in the wells but it is not a necessity to read the wells.
The reason why some kits mention it and others don't I've no clue.
Ockham's razor
Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".

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