Hi,
I need to prepare my cells for transplantation and need a final concentration of 13000 cells/µl. I centrifuged a 10 ml flask, took off the supernatant, and left only 1 ml. After counting the cells with a hematocytometer I ended up with 1000000 cells/ml or 1000 cells/µl. Does that mean that I have to reduce the volume of the media to 76 µl to get to 1000 cells/µl? I used the formula:
C1V1=C2V2
1000 cells/µl x 1 ml (1000 µl)= 13000 cells/µl X V2
V2= 77 µl
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#2
Dr Teeth
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Posted 06 August 2009 - 10:49 AM
mntl, on Aug 6 2009, 01:49 PM, said:
Hi,
I need to prepare my cells for transplantation and need a final concentration of 13000 cells/µl. I centrifuged a 10 ml flask, took off the supernatant, and left only 1 ml. After counting the cells with a hematocytometer I ended up with 1000000 cells/ml or 1000 cells/µl. Does that mean that I have to reduce the volume of the media to 76 µl to get to 1000 cells/µl? I used the formula:
C1V1=C2V2
1000 cells/µl x 1 ml (1000 µl)= 13000 cells/µl X V2
V2= 77 µl
I need to prepare my cells for transplantation and need a final concentration of 13000 cells/µl. I centrifuged a 10 ml flask, took off the supernatant, and left only 1 ml. After counting the cells with a hematocytometer I ended up with 1000000 cells/ml or 1000 cells/µl. Does that mean that I have to reduce the volume of the media to 76 µl to get to 1000 cells/µl? I used the formula:
C1V1=C2V2
1000 cells/µl x 1 ml (1000 µl)= 13000 cells/µl X V2
V2= 77 µl
Yes, 77 ul, rather than 1000, will yield 13,000 cells/ul.
Science is simply common sense at its best that is rigidly accurate in observation and merciless to fallacy in logic.
Thomas Henry Huxley
#3
mntl
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Posted 06 August 2009 - 11:31 AM
Dr Teeth, on Aug 6 2009, 11:49 AM, said:
mntl, on Aug 6 2009, 01:49 PM, said:
Hi,
I need to prepare my cells for transplantation and need a final concentration of 13000 cells/µl. I centrifuged a 10 ml flask, took off the supernatant, and left only 1 ml. After counting the cells with a hematocytometer I ended up with 1000000 cells/ml or 1000 cells/µl. Does that mean that I have to reduce the volume of the media to 76 µl to get to 1000 cells/µl? I used the formula:
C1V1=C2V2
1000 cells/µl x 1 ml (1000 µl)= 13000 cells/µl X V2
V2= 77 µl
I need to prepare my cells for transplantation and need a final concentration of 13000 cells/µl. I centrifuged a 10 ml flask, took off the supernatant, and left only 1 ml. After counting the cells with a hematocytometer I ended up with 1000000 cells/ml or 1000 cells/µl. Does that mean that I have to reduce the volume of the media to 76 µl to get to 1000 cells/µl? I used the formula:
C1V1=C2V2
1000 cells/µl x 1 ml (1000 µl)= 13000 cells/µl X V2
V2= 77 µl
Yes, 77 ul, rather than 1000, will yield 13,000 cells/ul.
Thanks.
