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Please, verify calculation


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#1 mntl

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Posted 06 August 2009 - 09:49 AM

Hi,
I need to prepare my cells for transplantation and need a final concentration of 13000 cells/Ál. I centrifuged a 10 ml flask, took off the supernatant, and left only 1 ml. After counting the cells with a hematocytometer I ended up with 1000000 cells/ml or 1000 cells/Ál. Does that mean that I have to reduce the volume of the media to 76 Ál to get to 1000 cells/Ál? I used the formula:
C1V1=C2V2
1000 cells/Ál x 1 ml (1000 Ál)= 13000 cells/Ál X V2
V2= 77 Ál

#2 Dr Teeth

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Posted 06 August 2009 - 10:49 AM

Hi,
I need to prepare my cells for transplantation and need a final concentration of 13000 cells/Ál. I centrifuged a 10 ml flask, took off the supernatant, and left only 1 ml. After counting the cells with a hematocytometer I ended up with 1000000 cells/ml or 1000 cells/Ál. Does that mean that I have to reduce the volume of the media to 76 Ál to get to 1000 cells/Ál? I used the formula:
C1V1=C2V2
1000 cells/Ál x 1 ml (1000 Ál)= 13000 cells/Ál X V2
V2= 77 Ál



Yes, 77 ul, rather than 1000, will yield 13,000 cells/ul.

Science is simply common sense at its best that is rigidly accurate in observation and merciless to fallacy in logic.
Thomas Henry Huxley

#3 mntl

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Posted 06 August 2009 - 11:31 AM

Hi,
I need to prepare my cells for transplantation and need a final concentration of 13000 cells/Ál. I centrifuged a 10 ml flask, took off the supernatant, and left only 1 ml. After counting the cells with a hematocytometer I ended up with 1000000 cells/ml or 1000 cells/Ál. Does that mean that I have to reduce the volume of the media to 76 Ál to get to 1000 cells/Ál? I used the formula:
C1V1=C2V2
1000 cells/Ál x 1 ml (1000 Ál)= 13000 cells/Ál X V2
V2= 77 Ál



Yes, 77 ul, rather than 1000, will yield 13,000 cells/ul.


Thanks.




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