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Couple questions about GST purification


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#16 Niraj

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Posted 02 September 2009 - 08:36 AM

[/quote]
Your normal lysis solution plus 10% glycerol. The ancient text known as my PhD thesis used this recipe:
100 mM Tris, pH7.5, 150 mM NaCl, 0.1 mM PMSF, 2% Triton X-100, 0.1% mercaptoethanol, 10% glycerol.
[/quote]
Hi Swanny,
I had used the above buffer for downstream washing of complex. And it is working great.
Thanks a lot.
Niraj

#17 swanny

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Posted 02 September 2009 - 10:53 PM

Excellent news! Glad I could help.
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