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Factor Xa secondary cleavage sites


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#1 pET

pET

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Posted 06 August 2009 - 01:15 AM

Dear all
I want to do expression of my protein in E.coli with a His Tag, to be able to cleave the tag afterwards I plan to insert a Factor XA cleavage site. At New England Biolabs they remark that a secondary cleavage site for Factor XA is Gly-Arg primarily when proteins are unfolded. I wonder now if anyone ever experienced that effect because Gly-Arg is a rather common motive in my opinion, and Factor XA a rather common used protease, also I did not find an indication for the secondary cleavage anywhere else.
Should I worry using the Factor XA in constructs with Gly-Arg now ?
pET




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