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UNIJET II and UNIVAPO ECH 150


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5 replies to this topic

#1 wntiong

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Posted 06 August 2009 - 12:29 AM

I need speedvac for my cDNA precipitation purpose, however my lab only has UNIJET II and UNIVAPO ECH 150 which is also a vacuum concentrator. Do you this equipment works the same function as speedvac? i neither use speedvac and this equipment before, but i got to learn how to use in order for my cDNA precipitation. Can gimme any advice? Thx

#2 mdfenko

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Posted 06 August 2009 - 09:48 AM

the univapo ech is similar to the speedvac.

the unijet ii is a vacuum pump. if it pulls a good enough vacuum then the combination is fine for your purpose.
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#3 wntiong

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Posted 17 August 2009 - 05:48 PM

Thanks mdfenko,

In fact i don't know how to operate this equipment. Any advise for beginner like me? like if i want to dry my RNA pellet, shd i use the default setting or manual setting? What parameter i shd look into? Thanks

#4 mdfenko

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Posted 17 August 2009 - 08:36 PM

my experience is with the speedvac.

leave the tubes open (or put a hole in the cap with a needle), close the lid, start the centrifuge then apply vacuum.

run until dry (or as close to dry as you want).
talent does what it can
genius does what it must
i do what i get paid to do

#5 wntiong

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Posted 18 August 2009 - 06:26 PM

Thanks. But there must be a setting for me to begin right? i totally no idea and there is no similar protocol/setting i can find on internet. Need help. Thanks!

#6 mdfenko

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Posted 21 August 2009 - 04:14 PM

you can try contacting the manufacturer and ask for a manual:
uniequip


or montreal biotech
talent does what it can
genius does what it must
i do what i get paid to do




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