multi well pippette tips-change or not
Posted 05 August 2009 - 11:39 PM
a small question but would like to get clarified,while using the multi well pippete for the wash steps in elisa,do i need to change the tips everytime,for eg in bethyl kit,they have recommended 5 times wash after each step,so do we need to change the pippette tips after each wash(96 wells x15-20 times totally for each elisa) or just use the same for all-samples and standards.how does everyone deal with it.
Posted 06 August 2009 - 12:39 AM
I wouldn't have thought you would need to change your tips, but then I haven't done an ELISA for ages and am definitely no expert
Posted 06 August 2009 - 12:41 AM
Posted 11 August 2009 - 12:59 PM
Another better and quicker option is to find a 8 or 12 well manifold and connect it to a bottle top dispensor you can then fill all wells evenly.