I have been running a few plates on our real time machine this week and I have a question for you all. I always run my samples in triplicates and use the average of the 3 numbers. Sometimes I will get very consistant results (ex. Ct values of 25.15, 25.17, 25.19). However, sometimes one of those numbers are way off, like 25.15, 25.17, 27.90. Can I exclude the last number when this is the case?
I have heard that many people go by different "rules." I've heard that people exclude nothing and take the average. I've heard that some people exclude one number if it is off by 0.5 compared to the other two. What is the RULE??
Thank you so much!! I'm new at this.
Edited by fungus9, 05 August 2009 - 06:42 PM.