Hi,
I am going to look at bacterial viability after ingestion by macrophages. I will lyse the cells and initially plate out the lysates to count cfu's
However, I need to do this at a high thoroughput level, and therefore would like to use a fluorescent/colormetric assay in 96 well plates to determine bacterial viability.
I have heard conflicting results regarding the alamar blue (resazurin) assay, and wonder if anyone has used similar assays? or even looked at fluorescent assays such as calcein?
Thanks!
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bacterial viability assay
Started by OKSO, Aug 05 2009 07:37 AM
4 replies to this topic
#2
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Posted 05 August 2009 - 09:15 AM
You can look into some DNA binding dye on fixed bacteria.
#3
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Posted 06 August 2009 - 01:50 AM
How do you propose to validate whatever technique you emply in this application?
#4
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Posted 06 August 2009 - 02:04 AM
I guess that's why I'm using this forum.
An obvious method would be correlation with cfu's, but am open to whatever has been tried and tested by others...
An obvious method would be correlation with cfu's, but am open to whatever has been tried and tested by others...
#5
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Posted 15 November 2009 - 01:36 AM
you can use for example SYTO-9 (live) and propidium iodide (dead) ...but i don't know how this interferse with your macrophages. I don't get it how you would do this in 96-well plates? How will you do the read out of such an assay? Maybe you can comment on that?
Regards,
p
Regards,
p
