bacterial viability assay
Posted 05 August 2009 - 07:37 AM
I am going to look at bacterial viability after ingestion by macrophages. I will lyse the cells and initially plate out the lysates to count cfu's
However, I need to do this at a high thoroughput level, and therefore would like to use a fluorescent/colormetric assay in 96 well plates to determine bacterial viability.
I have heard conflicting results regarding the alamar blue (resazurin) assay, and wonder if anyone has used similar assays? or even looked at fluorescent assays such as calcein?
Posted 05 August 2009 - 09:15 AM
Posted 06 August 2009 - 01:50 AM
Posted 06 August 2009 - 02:04 AM
An obvious method would be correlation with cfu's, but am open to whatever has been tried and tested by others...
Posted 15 November 2009 - 01:36 AM