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sjTREC real time PCR


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#1 meticulous me

meticulous me

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Posted 05 August 2009 - 07:10 AM

I am quantitating sjTRECs in patient and NL control samples using real time PCR. My problem is that the DNA I am using was not sorted for T cells, and I need to control for the amount of T cells. Is there any type of reference gene I can use to standardize for the amount of T cells? I tried TCRalpha but it was amplified in non T cell lines.




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