I'm looking for alternative ways to calculate the efficiency of of my primers.
I found an article describing a method for quantifying qRT-PCR results using calculations based on the kinetics of individual PCR reactions:
[Sheng Zhao, Russell D. Fernald. Comprehensive algorithm for quantitative real-time polymerase chain reaction. J. Comput. Biol. 2005 Oct;12(8):1045-62]
They use the raw data (no baseline substraction) to calculate efficiency and Ct for each single well.
Does anyone have experience whith this method or know other methods that do the same and give relyable results?
Thanks for your imput!
Real-time PCR Miner
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