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[rabos]

hi ,
i do not have Gndn- Cl (denaturant) which is prescribed in protein washing step using trizol[ .3M Soln in 95% abs. ethanol]. my lab has guanidine isothiocynate, urea , 2 mercaptoethanol etc, can i use any of these as substitute. my ultracentrifuge is out of order  so trizol is my only option .. besides i regularly  get rna for my rt pcr using trizol... just starting with protein work ..
                plse help

[stardust]

Hi,

I can't help with your question but wanted to mention, that I was never happy with the quality of protein from Trizol preparations...never dissolved properly after drying and all Western Blots looked weird. I would choose a different method for protein isolation.

Stardust