Posted 03 August 2009 - 01:40 PM
Just a quick question. I have started doing a lot of ELISA 's and when I graph the raw data, I am getting pg/ml values in the 10000-100000. The ELISA template I am using is from another lab and this is what they use to graph their results. I have doubled checked that I am using the template correctly and I am but I am still worried about my fold values.
I have looked up journals and do not see any papers publishing ELISA graphs with such high values. My cells are HEK293, my activator is poly I:C and my ELISA kit is from R+D systems. I would appreciate any help.
Thank you in advance.
Posted 04 August 2009 - 01:42 AM
Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".
Posted 04 August 2009 - 05:28 AM
Posted 06 August 2009 - 11:06 AM