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Low pH Native gel


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#1 anonymous

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Posted 23 August 2001 - 09:00 PM

I have a protein with a pi of 8.4. This is close to the pH of the gel and the protein hangs at the beginning of the gel. If I make the pH of the gel much higher, it wont buffer and and the protein will still not run. Does anyone know a protocol for a low ph native gel?

#2 Barbara

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Posted 02 November 2001 - 10:00 PM

Analytical Biochemistry 126, 94-99 (1982)






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