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Low pH Native gel
1 reply to this topic
Posted 23 August 2001 - 09:00 PM
I have a protein with a pi of 8.4. This is close to the pH of the gel and the protein hangs at the beginning of the gel. If I make the pH of the gel much higher, it wont buffer and and the protein will still not run. Does anyone know a protocol for a low ph native gel?