2 replies to this topic

[tatzilo]

Hi,

up to now we found a lot of ideas how to fix suspension cells onto a surface. The best seems to be working with 4% PFA.
But when it comes to carry out the experiment a few questions arose:

- centrifugation or waiting to settle down the cells
- how to apply PFA not to bring the cells into suspension

Thanks in advance :)

[cellcounter]

tatzilo, on Aug 3 2009, 06:58 AM, said:

Hi,

up to now we found a lot of ideas how to fix suspension cells onto a surface. The best seems to be working with 4% PFA.
But when it comes to carry out the experiment a few questions arose:

- centrifugation or waiting to settle down the cells
- how to apply PFA not to bring the cells into suspension

Thanks in advance

You can try out cytospin or frozen sections of agar-embedded block. See protocols here.

[little mouse]

If you don't have a cytospin, you can let the cells settle down on a polylysin coated glass slide. wash, do your labeling and fix. I did it with CD8+ T cells.