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in vitro siRNA


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#1 hclbs

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Posted 03 August 2009 - 06:44 AM

IŽll buy a siRNA for in vitro assay, and have doubts about the right brand...
Has anyone used siRNA for Akt1? Has it worked out?
thk

#2 cellcounter

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Posted 03 August 2009 - 08:23 AM

IŽll buy a siRNA for in vitro assay, and have doubts about the right brand...
Has anyone used siRNA for Akt1? Has it worked out?
thk

I had good experience with Dharmacon siRNA oligos (now Thermo Scientific). If you are thinking of shRNA vectors, I had good experience with pSingle inducible vector from Clontech. No experience with Akt1.

#3 Jojo

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Posted 13 November 2009 - 10:48 AM

Hi! So you have experience with the pSingle-tTS-shRNA from Clontech?
I am looking for an inducible system to use and I was thinking about this one. Do you think it is a good choice?
But it is really expensive!!! They are asking me about 1500 euros to get it! Is that normal?!
Thanks thanks!
Jojo




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