IŽll buy a siRNA for in vitro assay, and have doubts about the right brand...
Has anyone used siRNA for Akt1? Has it worked out?
thk
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in vitro siRNA
Started by hclbs, Aug 03 2009 06:44 AM
2 replies to this topic
#2
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Posted 03 August 2009 - 08:23 AM
hclbs, on Aug 3 2009, 06:44 AM, said:
IŽll buy a siRNA for in vitro assay, and have doubts about the right brand...
Has anyone used siRNA for Akt1? Has it worked out?
thk
Has anyone used siRNA for Akt1? Has it worked out?
thk
I had good experience with Dharmacon siRNA oligos (now Thermo Scientific). If you are thinking of shRNA vectors, I had good experience with pSingle inducible vector from Clontech. No experience with Akt1.
#3
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Posted 13 November 2009 - 10:48 AM
Hi! So you have experience with the pSingle-tTS-shRNA from Clontech?
I am looking for an inducible system to use and I was thinking about this one. Do you think it is a good choice?
But it is really expensive!!! They are asking me about 1500 euros to get it! Is that normal?!
Thanks thanks!
Jojo
I am looking for an inducible system to use and I was thinking about this one. Do you think it is a good choice?
But it is really expensive!!! They are asking me about 1500 euros to get it! Is that normal?!
Thanks thanks!
Jojo
