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cloudy gel


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#1 parinaz

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Posted 02 August 2009 - 01:47 AM

I have prepared a 15% gel but after polymerization it bocomes cloudy what should I do?

#2 bob1

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Posted 02 August 2009 - 04:20 PM

Determine what it is that is making it cloudy, I am guessing precipitation of something. Prepare fresh solutions and try again.

#3 mdfenko

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Posted 03 August 2009 - 08:38 AM

did you prepare all the solutions including acrylamide?

did you add the right amount of bisacrylamide to the acrylamide solution?

a relatively high percentage of bisacrylamide will turn the gel cloudy when polymerized.
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