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Histology Help


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#1 armoore

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Posted 31 July 2009 - 03:03 PM

I have mouse knees that have been fixed in formalin and decalcified in EDTA. My lab has done some staining on sections from these samples. I have some more knee samples from a while back that have been flash frozen and are stored in liquid nitrogen. Is there anyway to fix and decalcify these samples for histology after they've been frozen?
Thanks!

#2 bob1

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Posted 05 August 2009 - 04:20 PM

Not really, the soft tissues will go to mush on thawing. If you are just looking at the bone, this might not be a problem.




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