I have mouse knees that have been fixed in formalin and decalcified in EDTA. My lab has done some staining on sections from these samples. I have some more knee samples from a while back that have been flash frozen and are stored in liquid nitrogen. Is there anyway to fix and decalcify these samples for histology after they've been frozen?
Thanks!
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bob1
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Thelymitra pulchella
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Posted 05 August 2009 - 04:20 PM
Not really, the soft tissues will go to mush on thawing. If you are just looking at the bone, this might not be a problem.
