Hello,
I'm sorry to bother with such a simple question, but does anyone know if lamda-pir DH5a is avalible from a company? I can't seem to find it anywhere and it seems like such a common thing. If anyone could help me ge ahold of this I'd really appricate it.
Thank you
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BIOKMST
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Posted 30 July 2009 - 06:52 AM
Ro-85, on Jul 30 2009, 06:28 AM, said:
Hello,
I'm sorry to bother with such a simple question, but does anyone know if lamda-pir DH5a is avalible from a company? I can't seem to find it anywhere and it seems like such a common thing. If anyone could help me ge ahold of this I'd really appricate it.
Thank you
I'm sorry to bother with such a simple question, but does anyone know if lamda-pir DH5a is avalible from a company? I can't seem to find it anywhere and it seems like such a common thing. If anyone could help me ge ahold of this I'd really appricate it.
Thank you
You can search for pGP704 or its derivative pCVD442 and insert these plasmids into your DH5alpha to get the suicide gene. They should be readily available from a number of commercial vendors.
C.
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fishdoc
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Posted 30 July 2009 - 07:08 AM
BIOKMST, on Jul 30 2009, 09:52 AM, said:
Ro-85, on Jul 30 2009, 06:28 AM, said:
Hello,
I'm sorry to bother with such a simple question, but does anyone know if lamda-pir DH5a is avalible from a company? I can't seem to find it anywhere and it seems like such a common thing. If anyone could help me ge ahold of this I'd really appricate it.
Thank you
I'm sorry to bother with such a simple question, but does anyone know if lamda-pir DH5a is avalible from a company? I can't seem to find it anywhere and it seems like such a common thing. If anyone could help me ge ahold of this I'd really appricate it.
Thank you
You can search for pGP704 or its derivative pCVD442 and insert these plasmids into your DH5alpha to get the suicide gene. They should be readily available from a number of commercial vendors.
C.
pGP704 is lambda pir-dependent, it does not carry the lambda pir gene.
If what you're looking for is simply a lambda pir strain, but not specifically DH5a, you can try SM10 lambda pir or S17-1 lambda pir.
In any event, in most cases you don't need to buy the strain from a company, you may be able to request a culture from someone you know, someone in the field, or contact someone that used it in a paper. There are also E. coli strain repositories you could contact to get a strain. It may cost $50 or $60 going that route...
One such source: http://cgsc.biology.yale.edu/index.php
Edited by fishdoc, 30 July 2009 - 07:13 AM.
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Ro-85
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Posted 30 July 2009 - 07:29 AM
fishdoc, on Jul 30 2009, 08:08 AM, said:
pGP704 is lambda pir-dependent, it does not carry the lambda pir gene.
If what you're looking for is simply a lambda pir strain, but not specifically DH5a, you can try SM10 lambda pir or S17-1 lambda pir.
In any event, in most cases you don't need to buy the strain from a company, you may be able to request a culture from someone you know, someone in the field, or contact someone that used it in a paper. There are also E. coli strain repositories you could contact to get a strain. It may cost $50 or $60 going that route...
One such source: http://cgsc.biology.yale.edu/index.php
If what you're looking for is simply a lambda pir strain, but not specifically DH5a, you can try SM10 lambda pir or S17-1 lambda pir.
In any event, in most cases you don't need to buy the strain from a company, you may be able to request a culture from someone you know, someone in the field, or contact someone that used it in a paper. There are also E. coli strain repositories you could contact to get a strain. It may cost $50 or $60 going that route...
One such source: http://cgsc.biology.yale.edu/index.php
I am looking specifically for a DH5a, but thank you for the link. I will certainly check there.
