Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

where can I find lamda-pir DH5a?


  • Please log in to reply
3 replies to this topic

#1 Ro-85

Ro-85

    member

  • Active Members
  • Pip
  • 5 posts
0
Neutral

Posted 30 July 2009 - 05:28 AM

Hello,
I'm sorry to bother with such a simple question, but does anyone know if lamda-pir DH5a is avalible from a company? I can't seem to find it anywhere and it seems like such a common thing. If anyone could help me ge ahold of this I'd really appricate it.
Thank you

#2 BIOKMST

BIOKMST

    member

  • Active Members
  • Pip
  • 14 posts
0
Neutral

Posted 30 July 2009 - 06:52 AM

Hello,
I'm sorry to bother with such a simple question, but does anyone know if lamda-pir DH5a is avalible from a company? I can't seem to find it anywhere and it seems like such a common thing. If anyone could help me ge ahold of this I'd really appricate it.
Thank you



You can search for pGP704 or its derivative pCVD442 and insert these plasmids into your DH5alpha to get the suicide gene. They should be readily available from a number of commercial vendors.

C.

#3 fishdoc

fishdoc

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 272 posts
2
Neutral

Posted 30 July 2009 - 07:08 AM

Hello,
I'm sorry to bother with such a simple question, but does anyone know if lamda-pir DH5a is avalible from a company? I can't seem to find it anywhere and it seems like such a common thing. If anyone could help me ge ahold of this I'd really appricate it.
Thank you



You can search for pGP704 or its derivative pCVD442 and insert these plasmids into your DH5alpha to get the suicide gene. They should be readily available from a number of commercial vendors.

C.



pGP704 is lambda pir-dependent, it does not carry the lambda pir gene.

If what you're looking for is simply a lambda pir strain, but not specifically DH5a, you can try SM10 lambda pir or S17-1 lambda pir.

In any event, in most cases you don't need to buy the strain from a company, you may be able to request a culture from someone you know, someone in the field, or contact someone that used it in a paper. There are also E. coli strain repositories you could contact to get a strain. It may cost $50 or $60 going that route...


One such source: http://cgsc.biology.yale.edu/index.php

Edited by fishdoc, 30 July 2009 - 07:13 AM.


#4 Ro-85

Ro-85

    member

  • Active Members
  • Pip
  • 5 posts
0
Neutral

Posted 30 July 2009 - 07:29 AM

pGP704 is lambda pir-dependent, it does not carry the lambda pir gene.

If what you're looking for is simply a lambda pir strain, but not specifically DH5a, you can try SM10 lambda pir or S17-1 lambda pir.

In any event, in most cases you don't need to buy the strain from a company, you may be able to request a culture from someone you know, someone in the field, or contact someone that used it in a paper. There are also E. coli strain repositories you could contact to get a strain. It may cost $50 or $60 going that route...


One such source: http://cgsc.biology.yale.edu/index.php



I am looking specifically for a DH5a, but thank you for the link. I will certainly check there.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.