Posted 30 July 2009 - 03:49 AM
Posted 30 July 2009 - 06:47 AM
I have frozen digestions overnight at -20 and ran gels the next day with successful cloning. Maybe if they are frozen they are still OK. But, when in doubt and preventing days of no results - take time and do another hour digestion....
Posted 30 July 2009 - 06:48 AM
Posted 30 July 2009 - 07:22 AM
Thus I assume that so long as the digested DNA fragment was prepared without contamination from nucleases, kept on ice/cool and when not in use frozen in TE at -20C, the fragment should remained ligatable for a good few years.