There is such a thing as 5 minute ligations and they are wonderful. Invitrogen sells a number of kits with this ligation procedure. The kits are all called TOPO-.... I am not very familiar with the mechanism, but it works really well.
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#2
anonymous
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Posted 31 October 2001 - 10:00 PM
Hi,everybody,how can i perform DNA ligation in five minutes?thanks
#3
anonymous
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Posted 04 November 2001 - 10:00 PM
Try T4 DNA ligase offered by Gibco. They claim 5 minutes-ligation at room temperature. No affiliation.
CUHKMBT.
