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about rapid ligation


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#1 anonymous

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Posted 31 October 2001 - 10:00 PM

There is such a thing as 5 minute ligations and they are wonderful. Invitrogen sells a number of kits with this ligation procedure. The kits are all called TOPO-.... I am not very familiar with the mechanism, but it works really well.

#2 anonymous

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Posted 31 October 2001 - 10:00 PM

Hi,everybody,how can i perform DNA ligation in five minutes?thanks

#3 anonymous

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Posted 04 November 2001 - 10:00 PM

Try T4 DNA ligase offered by Gibco. They claim 5 minutes-ligation at room temperature. No affiliation.

CUHKMBT.





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