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[nicolo]

Hi,

I have a problem in studying DNA damage by using TUNEL assay (by flow cytometry). The positive control is treated with 2U of DNase. However, the result showed no different to the negative control. I have try different fixaton and permeablization method. But still can’t solve the problem.

Actually, which is the most critical step in doing TUNEL labeling? I even can't find out the problem...

Give hand plz!!!