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Qiagen DNAeasy blood&tissue DNA isolation kit


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#1 YVETTE

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Posted 29 July 2009 - 02:01 AM

Is there anyone use Qiagen DNAeasy blood&tissue DNA isolation kit? I was useing this kit to isolate DNA from the tail of mice for genotyping. I had a lot of problem with the genotyping PCR these days. I changed the PCR reagents, but still don't work. Then I measured the DNA concentration and found that the concentration is as low as 20ng per microlitle.
I am asking if anyone here had the same bad experience and any of your suggestion will be appreciated.
thanks

#2 Arqwen

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Posted 06 August 2009 - 02:51 AM

How old is the kit? Are any of the buffers out of date / stored at the wrong temperature / precipitated?
Also I find the recommended final elution step can be too high, I only use 30ul as a standard




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