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Making standard dilution for qPCR - PCR, RT-PCR and Real-Time PCR - BioForum

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Making standard dilution for qPCR


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#1 Kidd

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Posted 28 July 2009 - 01:18 AM

Hi, all

I found one article about qPCR. In that article, the authors made 10-fold serial dilution of their plasmid control with nuclease-free water containing carrier tRNA (100 ng/microliter)

Is cRNA critical for making serial dilution?

If anyone has information about cRNA for making DNA serial dilution, please share your information.



Here is the link to that article.

http://www.pubmedcen...bmedid=15933012

#2 dreww

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Posted 29 July 2009 - 04:08 AM

thank god, Im doing qPCR and RT-PCR and any literature is great! sorry i was no help




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