Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Help with Hep G2 cells

  • Please log in to reply
1 reply to this topic

#1 Katie Hulse

Katie Hulse


  • Members
  • Pip
  • 2 posts

Posted 27 July 2009 - 12:40 PM

Could anyone provide some suggestions for working with HepG2 cells? I have some cells that were frozen by a previous lab member. They are at passage 76. When I thaw them and culture according to ATCC, most of the cells are floating by the next day. I have had the cells growing for over two weeks now, and have some clumps of cells, but nothing is spreading out on the plate, and I don't think that they will ever reach confluencey. Is the passage number too high? Would I be better off ordering new cells from ATCC? Any suggestions would be greatly appreciated. Thanks!


#2 bob1


    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,459 posts

Posted 27 July 2009 - 05:04 PM

I suspect the cells will not survive; those clumps you are seeing will likely be quite different to the parent line. You will be better off getting some more.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.