store whole blood with tri-reagent too long caused RNA degradation or poor RNA q
Posted 26 July 2009 - 07:53 PM
Posted 27 July 2009 - 06:02 AM
Just wonder if i freeze my whole blood containing tri-reagent in -80 for about 1 or 2 months can cause degradation of rna or poorer quality? can't isolate from fresh due to certain unavoidable reason. thanks.
That should be fine. RNA is stable at -80, and tri-reagent inhibits RNAse activity. I store tissue in trizol at -80 all the time.
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