Now on the western I cut the membrane in half and probed for Actin on the bottom and NRSF on the top. The bands all look nice, the 4-100% versus the 4-300% looks like it should for actin.
But the NRSF band for 4-300% is WAY lower signal than the 4-100%? What the!? What am I missing here, how is that even possible?
Any ideas? How can I trust my WB?
Edited by MKR, 24 July 2009 - 02:13 PM.