Edited by cbraval, 24 July 2009 - 09:21 AM.
opti-mem as a serum starvation media
1 reply to this topic
Posted 24 July 2009 - 09:21 AM
Hi can I use Opti-MEM as a media for serum starvation? I need to grow monolayers but want to maximize a drug treatment so I am starving. Any thoughts would be appreciated.
Posted 26 July 2009 - 03:52 PM
Use the same medium as you normally culture your cells in, just leave out the serum. Changing the type of medium on many cell lines causes them some stress, so it is best to keep things consistent.