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Making ampicillin solution


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5 replies to this topic

#1 miraclestrain

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Posted 23 July 2009 - 10:30 PM

Hi all,

I am trying to dissolve ampicillin trihydrate in distilled water at a concentration of 50mg/ml.

I wanted to prepare 50ml of ampicillin solution. So I used 2.5gm of ampicillin to dissolve in distilled water.I made it upto 50 ml.

But I have difficulty dissolving it. It remains as a ilky white solution and after few mins I can see the precipitation (remains insoluble).

Is there a solution for this?

Regards
MS

#2 T C

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Posted 24 July 2009 - 01:03 AM

I the ampicillin stock too old?

Hi all,

I am trying to dissolve ampicillin trihydrate in distilled water at a concentration of 50mg/ml.

I wanted to prepare 50ml of ampicillin solution. So I used 2.5gm of ampicillin to dissolve in distilled water.I made it upto 50 ml.

But I have difficulty dissolving it. It remains as a ilky white solution and after few mins I can see the precipitation (remains insoluble).

Is there a solution for this?

Regards
MS



#3 genehunter

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Posted 24 July 2009 - 05:44 AM

Have you adjusted the pH see if that helps?

#4 phage434

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Posted 24 July 2009 - 08:22 PM

Normally the sodium salt is used in making stock solutions. The trihydrate is soluble only at 10 mg/ml, while the sodium salt is soluble at more than 50 mg/ml. Add NaOH to form the sodium salt and it should go into solution. Next time order the sodium salt.

#5 miraclestrain

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Posted 28 July 2009 - 12:39 AM

Hi there,

Thanks, it did work as you told, can you be bit more specific about the sodium salt? Say if you were given ampicillin trihydrate what will add to make a concentration of 50mg/ml?

thanks

#6 phage434

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Posted 28 July 2009 - 06:36 PM

1 mole of ampicillin trihydrate needs 1 mole of NaOH to form the sodium salt. Determine the molarity of the 50 mg/ml solution (I don't off hand know the MW of ampicillin trihydrate), and add sufficient 1 M NaOH solution to form the sodium salt. Do this at below final volume, and top up the volume to its final value.




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