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How long are cells viable in PBS?


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#1 Penguin

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Posted 23 July 2009 - 07:41 AM

Hi,

I am going to have to do a lot of cell counts soon which I find incredibly boring. Once I've lifted the cells with trypsin, spun down and resuspended them in PBS, how long will they be viable for before staining with trypan blue?

I guess what I'm asking is can I do the cell counts in batches i.e. count for half an hour, go off and do something else for a bit then come back and do more counts, or do I have to sit there for a couple of hours and do them all in one go?

Thanks,
P

#2 cellcounter

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Posted 23 July 2009 - 12:08 PM

Hi,

I am going to have to do a lot of cell counts soon which I find incredibly boring. Once I've lifted the cells with trypsin, spun down and resuspended them in PBS, how long will they be viable for before staining with trypan blue?

I guess what I'm asking is can I do the cell counts in batches i.e. count for half an hour, go off and do something else for a bit then come back and do more counts, or do I have to sit there for a couple of hours and do them all in one go?

Thanks,
P

For a consistency you need to do them at one go. But if intersample consistency is not that important, you can do them in batches.

Every minute that the cells are left in PBS, a certain number of them die. It depends upon what cells you are using that how fast a significant numbers of them go dead.

In any case, do the following.

1. Keep the cells on ice.

1. Resuspend them in culture media, rather than the PBS.

This two measures will make the cells survive longer.

#3 Penguin

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Posted 24 July 2009 - 01:19 AM

For a consistency you need to do them at one go. But if intersample consistency is not that important, you can do them in batches.

Every minute that the cells are left in PBS, a certain number of them die. It depends upon what cells you are using that how fast a significant numbers of them go dead.

In any case, do the following.

1. Keep the cells on ice.

1. Resuspend them in culture media, rather than the PBS.

This two measures will make the cells survive longer.


Yeah, thats what I was thinking, I guess I'll just have to get on with it!! It won't be so bad if I have the radio on!
Thanks,
P

#4 ASHWANI

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Posted 12 August 2019 - 04:57 AM

The best thing as suggested by the other person is to keep cells in media. You can re-suspend the cells in plain media and keep for a longer time. But keep them in ice otherwise it wont work well.

Also, it will help you in better cell counting due to lesser cell death and debris thereof. 






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