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designing primers


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#1 Christina

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Posted 23 July 2009 - 04:58 AM

Dear all,
Could anyone give me some advice about how to design good primers for the target sequence. I plan to carry bisulfite sequencing and need good primers. I design many pairs of primers but I could not get any good result. it seems as a difficult task.I'm worried about my job,I wish somebody could help me solving my problem,thanks. here my sequence,anybody can help me designing any primers?yellow words is the part of amplifing.
I am looking forward to your answers!

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Edited by Christina, 23 July 2009 - 05:03 AM.


#2 RKA

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Posted 29 July 2009 - 01:09 AM

Dear all,
Could anyone give me some advice about how to design good primers for the target sequence. I plan to carry bisulfite sequencing and need good primers. I design many pairs of primers but I could not get any good result. it seems as a difficult task.I'm worried about my job,I wish somebody could help me solving my problem,thanks. here my sequence,anybody can help me designing any primers?yellow words is the part of amplifing.
I am looking forward to your answers!


you can use methyla primer express, can just paste your target sequence and get the primers for BSP or MSP




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