Could anyone give me some advice about how to design good primers for the target sequence. I plan to carry bisulfite sequencing and need good primers. I design many pairs of primers but I could not get any good result. it seems as a difficult task.I'm worried about my job,I wish somebody could help me solving my problem,thanks. here my sequence,anybody can help me designing any primers?yellow words is the part of amplifing.
I am looking forward to your answers!
Edited by Christina, 23 July 2009 - 05:03 AM.