1 reply to this topic

[Christina]

Dear all,
    Could anyone give me some advice about how to design good primers for the target sequence. I plan to carry bisulfite sequencing and need good primers. I design many pairs of primers but I could not get any good result. it seems as a difficult task.I'm worried about my job,I wish somebody could help me solving my problem,thanks. here my sequence,anybody can help me designing any primers?yellow words  is the part of amplifing.
    I am looking forward to your answers!

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Edited by Christina, 23 July 2009 - 05:03 AM.

[RKA]

Christina, on Jul 23 2009, 02:58 PM, said:

Dear all,
    Could anyone give me some advice about how to design good primers for the target sequence. I plan to carry bisulfite sequencing and need good primers. I design many pairs of primers but I could not get any good result. it seems as a difficult task.I'm worried about my job,I wish somebody could help me solving my problem,thanks. here my sequence,anybody can help me designing any primers?yellow words  is the part of amplifing.
    I am looking forward to your answers!

you can use methyla primer express, can just paste your target sequence and get the primers for BSP or MSP