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Multiplex PCR primer design


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#1 hobglobin

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Posted 23 July 2009 - 12:11 AM

Dear all, does anybody know a good program for designing or controlling primers for a multiplex pcr?
I found MulitPLX 2.0 but it cannot design the primers but just makes more or less good groups out of compatible self-designed primers.
Thanks in advance.

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#2 Superman

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Posted 24 July 2009 - 03:50 AM

Dear all, does anybody know a good program for designing or controlling primers for a multiplex pcr?
I found MulitPLX 2.0 but it cannot design the primers but just makes more or less good groups out of compatible self-designed primers.
Thanks in advance.


Hi,

Im not sure if I can help but you could try this.

universalprobelibrary.com

If the link does not work then google Universal Probe Library

Click on 'design real time q pcr primers' then follow on from there. you can select multiplex as you go along.

Hope it helped.

Edited by Superman, 24 July 2009 - 03:50 AM.





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