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Neutral loss Experiment, Acetaminophen Turnover


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#1 jummykorn

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Neutral

Posted 22 July 2009 - 09:32 AM

Having a problem seeing any turnover in Acetominophen in a neutral loss experiment.
Acetominophen incubuated with microsomes + 3 mixes

mix 1- GSH + MgCl2 +NADPH regenerating system
mix 2- GSH + MgCl2
mix 3- NADPH regenerating system

( no turnover is expected in mix 2)

Verapmil was also run with the same stock solutions and turnover was evident :D

What could be the cause of no to little turnover in Acetaminophen? ;)

#2 BIOKMST

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Neutral

Posted 30 July 2009 - 06:44 AM

Having a problem seeing any turnover in Acetominophen in a neutral loss experiment.
Acetominophen incubuated with microsomes + 3 mixes

mix 1- GSH + MgCl2 +NADPH regenerating system
mix 2- GSH + MgCl2
mix 3- NADPH regenerating system

( no turnover is expected in mix 2)

Verapmil was also run with the same stock solutions and turnover was evident :)

What could be the cause of no to little turnover in Acetaminophen? :)


Hi, possibly an irreversible inhibition with microsome binding?




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