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[jummykorn]

Having a problem seeing any turnover in Acetominophen in a neutral loss experiment.
Acetominophen incubuated with microsomes + 3 mixes

mix  1- GSH + MgCl2 +NADPH regenerating system
mix  2- GSH + MgCl2
mix  3- NADPH regenerating system

( no turnover is expected in mix 2)

Verapmil was also run with the same stock solutions and turnover was evident

What could be the cause of no to little turnover in Acetaminophen?

[BIOKMST]

jummykorn, on Jul 22 2009, 10:32 AM, said:

Having a problem seeing any turnover in Acetominophen in a neutral loss experiment.
Acetominophen incubuated with microsomes + 3 mixes

mix  1- GSH + MgCl2 +NADPH regenerating system
mix  2- GSH + MgCl2
mix  3- NADPH regenerating system

( no turnover is expected in mix 2)

Verapmil was also run with the same stock solutions and turnover was evident

What could be the cause of no to little turnover in Acetaminophen?

Hi, possibly an irreversible inhibition with microsome binding?