Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

RNA electrophoresis


  • Please log in to reply
2 replies to this topic

#1 ningqun

ningqun

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 06 July 2002 - 04:28 PM

RNA was extracted from the brain tissue in order to northern blot, then electrophoresis was explored to exam the integrality of RNA. when the RNA was treated with formamide, formaldahydel and loading dye (xylene cyanole, bromophenol blue, glycerol DEPC water) to degeneration, no bands was showed. When RNA was only treated with loading dye, 3 bands(5s, 18s, 28s) could be observed clearly. There's some problems with formamide, formaldahydel ? How can I solve this problem.

help! helP! sos!sos!

#2 misscrews

misscrews

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 10 July 2002 - 10:36 AM

Try using 4.0 ul of 5X MAE, 3.5 ul of (37%) formaldehyde, 10.0 ul of formamide, and 1.0 ul of EtBr (5 mg/ul) per tube (with about 4.5 ul of your RNA sample.  Incubate at 55 degrees C for 15 minutes then add 6X loading dye to each sample and load onto your gel.  

Are you storing your samples on ice while mixing everything?  How much of the Formaldehyde and formamide are you using?  Are you running the samples on a FA gel?  

#3 Gonzo

Gonzo

    member

  • Active Members
  • Pip
  • 9 posts
0
Neutral

Posted 20 January 2010 - 07:11 AM

Exactly the same with me. Did you solve the problem?




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.