42968 replies to this topic

[casandra]

Conge

hey swanny...we still have a lot of snow on the ground but with a high of +1 then it was an almost a perfect winter day Have you broken all your resolutions already?

[swanny]

Bungee

+1!!! I don't get out of bed for an expected maximum less than 15!

What is this "resolution" you speak of? Some new-fangled Christmas toy for grown-ups?

[casandra]

Jump

hey guys...hi swanny..so are you getting addicted to the cold now? Then join the club... and yup, resolutions-those which are custom-made just to be broken...and I broke one just last night..still have all the scars to prove it.....

[hobglobin]

hump

Hi people and hi Mlle C...well a good idea not to know what resolutions are, then you don't make some

[Tabaluga]

hungry

Hi everyone ! My resolution for this year is the same as for last year:1280x720.

[casandra]

ravenous

then we would like proof of this.......hey Tabs....howz the sticky cells situation?

[Tabaluga]

raven

Didn't get round to try out today, unfortunately (spent half the day phoning around for a formal document I need, only to be put off to next month in the end, and the other half collecting and formatting microscopic images....) But i'm really curious, will try tomorrow!

[casandra]

ravine

Yup, work sucks......btw, I asked a neighboring lab and they said they use Ambion tubes- they said they're not that expensive but you know, cost is relative...for a rich lab, they would say they're not expensive.... you might have to do some shopping though...

[Tabaluga]

ravioli

OK, thanks for the info !! I'll see what can be done about it. But it's really a bit embarassing if you are the only person in the lab having the problem , and then ask for more expensive material to solve it.

Anyway, I wish you a good night, casandra !

[swanny]

Ravishing

Sorry for asking for a big rehash, but what's the problem with your cells being sticky?

[Tabaluga]

relish

Hi swanny. My cells tend to stick on the walls of the reaction tube when I centrifuge them, meaning I lose a part of them at every centrifugation step (and I've often got many centrifugation steps for one experiment). We're discussing the problem in this thread: http://www.protocol-...be/#entry147699

Edited by Tabaluga, 09 January 2013 - 07:41 PM.

[casandra]

hotdog

hi Tabs (you're still awake? Or you're not living across the pond anymore?)...hey swanny, your temps are off the chart already?

[Tabaluga]

dogtanian

Hi casandra ! Yes, I was still awake then (the dear microscopic pictures again)

Edited by Tabaluga, 10 January 2013 - 05:37 AM.

[casandra]

pomeranian

hey guys...hi Tabs, give the images a rest then and it's better to get more beauty sleep

[hobglobin]

portmanteau

Hi people