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Bacteria Changing Colour


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#1 microbes

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Posted 20 July 2009 - 11:09 PM

hello everybody,
i have a bacteria that is gram negative cocci. special thing about it is that it changes colour with growth, means growing freshly on nutrient agar its Red and then turns Cream colour with time, but again it is Red at the outer ends of colony means where it has access to media and growing fresh there,
So can anybody tell me more about this....

Arvind Behal

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#2 LordPhantom

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Posted 21 July 2009 - 12:22 AM

This reminds me of Serratia marcescens. It's has a pinkish red pigmentation when it grows due to prodigiosin. Usually bacteria display discoloration under certain environmental condition, such as cell density in the colony. This might explain why it changes from red to cream color over time. Although, I doubt what you plated is S. marcescens as it is a bacillus instead of a cocci... ;)

#3 GeorgeWolff

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Posted 21 July 2009 - 03:11 AM

Serratia marscens doesn't usually show that degree of color diversity. How fast do the colonies appear and does it ferment glucose and show bile resitance - grow on such media as MacConkey and EMB? Could you send us a picture?

#4 microbes

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Posted 21 July 2009 - 08:57 PM

hello
I am working on it and soon will post the pics of colony as well as stained slide, and biochemicals tests will take sme time also.
thanks
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#5 microbes

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Posted 31 August 2009 - 12:58 AM

here is a pic of bacteria, they are gram negative small coccus bacteria

Attached File(s)


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#6 GeorgeWolff

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Posted 31 August 2009 - 12:58 PM

Thanks - I see no change in color here and pigmentation sure looks like Serratia marcescens.

In any case, pigmentation is not an end point in identification - can you not complete that?

Edited by GeorgeWolff, 31 August 2009 - 12:58 PM.


#7 microbes

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Posted 06 September 2009 - 07:31 PM

hello
can you please suggest me further tests i should follow for it.
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#8 eberthella

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Posted 08 September 2009 - 01:41 AM

Look at "unidentified soil bacterium" discussion here in microbiology.





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