lactose tetrazolium

Started by arvinsign, Jul 20 2009 10:51 PM

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#1 arvinsign

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Posted 20 July 2009 - 10:51 PM

does anyone here have experience in the use of lactose tetrazolium agar? can you please share your recipe? Thanks

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#2 GeorgeWolff

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Posted 21 July 2009 - 03:28 AM

There's a good discussion of its application at : NATURE REVIEWS | GENETICS VOLUME 4 | JUNE 2003 | 419 ART AND DESIGN OF GENETIC SCREENS: ESCHERICHIA COLI

Ref for prep.: Beckwith, J. Restoration of operon activity by suppressors. Biochim. Biophys. Acta 76, 162–164 (1963).

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#3 arvinsign

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Posted 23 July 2009 - 01:16 AM

Thanks. i have that paper, however there was no mention of the recipe...just the principles. The Beckwith paper is not available in PDF

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#4 GeorgeWolff

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Posted 23 July 2009 - 03:42 AM

Contact Bruce Levin - think he's at Emory now.

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