3 replies to this topic

[arvinsign]

does anyone here have experience in the use of lactose tetrazolium agar? can you please share your recipe? Thanks

[GeorgeWolff]

There's a good discussion of its application at : NATURE REVIEWS | GENETICS VOLUME 4 | JUNE 2003 | 419 ART AND DESIGN OF GENETIC SCREENS: ESCHERICHIA COLI

Ref for prep.:  Beckwith, J. Restoration of operon activity by suppressors.  Biochim. Biophys. Acta 76, 162–164 (1963).

[arvinsign]

Thanks. i have that paper, however there was no mention of the recipe...just the principles. The Beckwith paper is not available in PDF

[GeorgeWolff]

Contact Bruce Levin - think he's at Emory now.