2X sample buffer vs. 6X
Posted 20 July 2009 - 04:40 PM
Posted 20 July 2009 - 05:25 PM
2X means you need to do a 2X dilution. So say.. you wanna load 10ul, that will be 10ul/2 = 5ul of your sample buffer to load to 5ul of your samples.
6X means a 6X dilution is required, so say... for 10ul, it will be 10ul/6 = 1.7ul of your sample buffer to load to 8.3ul of your samples.
Posted 20 July 2009 - 06:55 PM
Posted 20 July 2009 - 07:40 PM
Posted 21 July 2009 - 11:07 PM
So, for example, your 2X buffer is 2 times more concentrated than a working concentration of the buffer.