Is it possible to stop an ELISA (HRP used as enzyme, OPD/H2O2 as the substrate) with concentrated HCL? I see many protocols calling for stopping with H2SO4, will there be any difference in OD values since both HCL and H2SO4 will make the solution very acidic?
Thanks!
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Posted 20 July 2009 - 11:13 AM
Yes you can, I've used both in different elisa's en didn't notice a difference.
Don't use concentrated HCl but 1 or 2 M HCL
Don't use concentrated HCl but 1 or 2 M HCL
Ockham's razor
Pluralitas non est ponenda sine necessitate
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Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".
