Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

ELISA Help-stopping


  • Please log in to reply
1 reply to this topic

#1 tholusc

tholusc

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 20 July 2009 - 09:41 AM

Is it possible to stop an ELISA (HRP used as enzyme, OPD/H2O2 as the substrate) with concentrated HCL? I see many protocols calling for stopping with H2SO4, will there be any difference in OD values since both HCL and H2SO4 will make the solution very acidic?

Thanks!

#2 Gerard

Gerard

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 124 posts
0
Neutral

Posted 20 July 2009 - 11:13 AM

Yes you can, I've used both in different elisa's en didn't notice a difference.
Don't use concentrated HCl but 1 or 2 M HCL
Ockham's razor
Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.